Metabolic Engineering of Escherichia coli for Poly(3-hydroxybutyrate) Production under Microaerobic Condition

Table 4

Summary of engineered E. coli for improving P3HB production under microaerobic condition.

E. coli	Description	Cell dry weight (g/L)	P3HB content (%)	P3HB concentration (g/L)	Mixed acid (g/L)	Yield (g P3HB/g glucose)
BW25113	Wild type	6.72	44.53	3.06	13.60	0.10
JW2294	pta knockout	7.75	64.31	4.98	16.60	0.17
BWapld	Mixed acid fermentation deficient	6.79	73.58	5.00	1.29	0.30
BWapld (pMCS2pflB)	Mixed acid fermentation deficient and pflB overexpression	5.88	84.43	4.96	1.72	0.27

All E. coli strains carried plasmid pWYC09, which harbored phaCAB operon under the control of P_adhE. Mixed acid referred the total amount of succinate, formate, acetate, and ethanol.