Guidelines for Optimisation of a Multiplex Oligonucleotide Ligation-PCR for Characterisation of Microbial Pathogens in a Microsphere Suspension Array

Figure 3

Boxplot of signal-to-noise ratios for different probe concentrations in the ligation mix. Results of one of 2 independent tests are presented, that is, results of 6 isolates × 3 conditions = 18 MOL-PCR assays (for each of the 3 conditions: with and . neg: expected negative results; pos: expected positive results; R: reference condition; : statistically significant difference with the reference condition.