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BioMed Research International
Volume 2015 (2015), Article ID 790170, 10 pages
http://dx.doi.org/10.1155/2015/790170
Research Article

Guidelines for Optimisation of a Multiplex Oligonucleotide Ligation-PCR for Characterisation of Microbial Pathogens in a Microsphere Suspension Array

1Department of Microbial and Molecular Systems, KU Leuven, Kasteelpark Arenberg 20, Bus 2460, 3001 Leuven, Belgium
2Platform Biotechnology and Molecular Biology, Scientific Institute of Public Health (WIV-ISP), Juliette Wytsmanstraat 14, 1050 Brussels, Belgium
3National Reference Centre for Salmonella and Shigella, Bacterial Diseases Division, Communicable and Infectious Diseases, Scientific Institute of Public Health (WIV-ISP), Juliette Wytsmanstraat 14, 1050 Brussels, Belgium
4Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, 9052 Ghent, Belgium
5Department of Information Technology, Ghent University, IMinds, Gaston Crommenlaan 8, 9050 Ghent, Belgium

Received 16 September 2014; Revised 5 January 2015; Accepted 12 January 2015

Academic Editor: Marco Fichera

Copyright © 2015 Véronique Wuyts et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplemental figures show the boxplots of signal-to-noise ratios of additional parameters. The supplemental data sets include the MFI values that were used to calculate the signal-to-noise ratios as background information to explain some of the results.

  1. Supplementary Material