(a)
(b)
Figure 3: PP2Acα deletion inhibited HSC activation. (a) Immunohistochemical analysis of α-SMA expression in liver tissues from the control and PP2Acα knockout mice at 5 weeks after the chronic CCl4 treatment. Magnification, ×100. Bar, 50 μm. The histogram shows the mean percentage of the α-SMA positive area per total liver area determined from five randomly selected fields. Values are means ± SD, . . (b) Western blot analyses of hepatic α-SMA expression in the control and PP2Acα knockout mice at 5 weeks after the chronic CCl4 treatment. Liver homogenates were probed with antibodies against α-SMA and β-actin, respectively.