The Kampo Medicine Yokukansan Decreases MicroRNA-18 Expression and Recovers Glucocorticoid Receptors Protein Expression in the Hypothalamus of Stressed Mice

Figure 4

GR protein expression in the PVN of the hypothalamus. (a) Immunohistochemical analysis of CRF and GR in the PVN of the hypothalamus 2 h after the end of stress exposure. Scale bar, 50 μm. (b) Measurements of merged cell numbers in the PVN region. Results are the means ± SEMs of at least four independent experiments. The cell counts were Cont, 1086; Stress, 804, and Stress + YKS, 1141. Cont: control mice; Stress (YKS−): acute stress-exposed mice; Stress (YKS+): 1.0 g/kg YKS pretreatment and stress-exposed mice; , Student’s t-test.

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