Beneficial Effect of Sugar Osmolytes on the Refolding of Guanidine Hydrochloride-Denatured Trehalose-6-phosphate Hydrolase from Bacillus licheniformis

Table 1

Effect of sugar osmolytes on the refolding of denatured BlTreA at 25^°C.

Osmolyte concentration (M)	Sorbitol				Sucrose				Trehalose
	I	II	III	IV	I	II	III	IV	I	II	III	IV
0.00	100	513.5	330.7	4.0	100	513.5	330.7	4.0	100	513.5	330.7	4.0
0.30	50.3	374.9	333.5	35.3	48.8	372.6	332.7	31.0	49.30	369.6	334.2	33.3
0.75	61.5	416.3	331.5	27.1	55.5	357.4	333.7	25.5	51.60	380.8	333.9	30.0
1.00	57.6	417.2	332.1	25.9	54.5	357.9	332.6	25.5	47.88	377.6	334.1	30.7
1.25	51.8	406.7	332.3	26.8	49.4	361.2	332.9	27.1	33.10	384.7	333.8	35.3

I: final refolding yield of BlTreA; II and III (nm): maximum intensity and peak position of the intrinsic fluorescence emission spectra of the refolded BlTreA in the presence of sugar osmolytes; IV: ANS fluorescence intensity of the refolded BlTreA in the presence of sugar osmolytes.