The Impact of Vitamin D₃ Supplementation on Mechanisms of Cell Calcium Signaling in Chronic Kidney Disease

Figure 6

The expression of surface P2X₇ receptors. (a) Representative flow cytometry histograms of PBMCs immunostained with primary antibody for the extracellular domain of the P2X₇ receptor (gray peak) and an isotype-matched control (IgG2a, white peak). (b) Immunofluorescence staining of P2X₇ receptors in plasma membrane using anti-P2X₇ (extracellular) antibody of lymphocytes visualised by fluorescence microscopy. Images represent surface P2X₇ receptors in four different samples. (c) Decreased expression of surface P2X₇ receptors after vitamin D₃ supplementation in a whole population of PBMCs (, ).

(a)

(b)

(c)