Research Article
Cloning and Characterization of Spliced Variants of the Porcine G Protein Coupled Receptor 120
Figure 5
Signaling properties of pGPR120 WT and V2 isoforms. HEK293T cells were transfected with pGPR120-WT or pGPR120-V2 expression plasmid together with the NFAT ((a), (c)) and SRE ((b), (d)) reporter systems plasmids for 24 h. TUG-891 and endogenous fatty acids (EPA, DHA, ALA, LA, and PLA) were used at the range of concentration from 10−9 to 10−4 M to treat the transfected cells with serum-free starvation for 6 h. The cells were harvested to measure the luciferase activity normalized to the Renilla values.
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