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BioMed Research International
Volume 2015, Article ID 815413, 8 pages
http://dx.doi.org/10.1155/2015/815413
Research Article

A Novel Aqueous Micellar Two-Phase System Composed of Surfactant and Sorbitol for Purification of Pectinase Enzyme from Psidium guajava and Recycling Phase Components

1Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia
2Halal Products Research Institute, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia

Received 5 December 2014; Revised 23 January 2015; Accepted 1 February 2015

Academic Editor: Pengjun Shi

Copyright © 2015 Mehrnoush Amid et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. Rito-Palomares, “Practical application of aqueous two-phase partition to process development for the recovery of biological products,” Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 807, no. 1, pp. 3–11, 2004. View at Publisher · View at Google Scholar · View at Scopus
  2. J. S. Becker, O. R. T. Thomas, and M. Franzreb, “Protein separation with magnetic adsorbents in micellar aqueous two-phase systems,” Separation and Purification Technology, vol. 65, no. 1, pp. 46–53, 2009. View at Publisher · View at Google Scholar · View at Scopus
  3. A. Chavez-Santoscoy, J. Benavides, W. Vermaas, and M. Rito-Palomares, “Application of aqueous two-phase systems for the potential extractive fermentation of cyanobacterial products,” Chemical Engineering & Technology, vol. 33, no. 1, pp. 177–182, 2010. View at Publisher · View at Google Scholar · View at Scopus
  4. O. K. Ashipala and Q. He, “Optimization of fibrinolytic enzyme production by Bacillus subtilis DC-2 in aqueous two-phase system (poly-ethylene glycol 4000 and sodium sulfate),” Bioresource Technology, vol. 99, no. 10, pp. 4112–4119, 2008. View at Publisher · View at Google Scholar · View at Scopus
  5. J. Persson, A. Kaul, and F. Tjerneld, “Polymer recycling in aqueous two-phase extractions using thermoseparating ethylene oxide-propylene oxide copolymers,” Journal of Chromatography B: Biomedical Sciences and Applications, vol. 743, no. 1-2, pp. 115–126, 2000. View at Publisher · View at Google Scholar · View at Scopus
  6. F. Luechau, T. C. Ling, and A. Lyddiatt, “Partition of plasmid DNA in polymer-salt aqueous two-phase systems,” Journal of Separation and Purification Technology, vol. 66, no. 2, pp. 397–404, 2009. View at Publisher · View at Google Scholar · View at Scopus
  7. M. Li, J.-W. Kim, and T. L. Peeples, “Amylase partitioning and extractive bioconversion of starch using thermoseparating aqueous two-phase systems,” Journal of Biotechnology, vol. 93, no. 1, pp. 15–26, 2002. View at Publisher · View at Google Scholar · View at Scopus
  8. W. Schnitzhofer, H.-J. Weber, M. Vršanská, P. Biely, A. Cavaco-Paulo, and G. M. Guebitz, “Purification and mechanistic characterisation of two polygalacturonases from Sclerotium rolfsii,” Journal of Enzyme and Microbial Technology, vol. 40, no. 7, pp. 1739–1747, 2007. View at Publisher · View at Google Scholar · View at Scopus
  9. R. S. Jayani, S. Saxena, and R. Gupta, “Microbial pectinolytic enzymes: a review,” Journal of Process Biochemistry, vol. 40, no. 9, pp. 2931–2944, 2005. View at Publisher · View at Google Scholar · View at Scopus
  10. A. Jiménez-Escrig, M. Rincón, R. Pulido, and F. Saura-Calixto, “Guava fruit (Psidium guajava L.) as a new source of antioxidant dietary fiber,” Journal of Agricultural and Food Chemistry, vol. 49, no. 11, pp. 5489–5493, 2001. View at Publisher · View at Google Scholar · View at Scopus
  11. G. Flores, S.-B. Wu, A. Negrin, and E. J. Kennelly, “Chemical composition and antioxidant activity of seven cultivars of guava (Psidium guajava) fruits,” Food Chemistry, vol. 170, pp. 327–335, 2015. View at Publisher · View at Google Scholar
  12. M. G. Antov, D. M. Pericin, and G. R. Dimi, “Cultivation of Polyporus squamosus for pectinase production in aqueous two-phase system containing sugar beet extraction waste,” Journal of Biotechnology, vol. 91, no. 1, pp. 83–87, 2001. View at Publisher · View at Google Scholar · View at Scopus
  13. H. Barbosa, N. K. H. Slater, and J. C. Marcos, “Protein quantification in the presence of poly(ethylene glycol) and dextran using the Bradford method,” Analytical Biochemistry, vol. 395, no. 1, pp. 108–110, 2009. View at Publisher · View at Google Scholar · View at Scopus
  14. S. Mohamed Ali, T. C. Ling, S. Muniandy, Y. S. Tan, J. Raman, and V. Sabaratnam, “Recovery and partial purification of fibrinolytic enzymes of Auricularia polytricha (Mont.) Sacc by an aqueous two-phase system,” Separation and Purification Technology, vol. 122, no. 1, pp. 359–366, 2014. View at Publisher · View at Google Scholar · View at Scopus
  15. V. Kaushik and Y. H. Roos, “Limonene encapsulation in freeze-drying of gum Arabic-sucrose-gelatin systems,” LWT: Food Science and Technology, vol. 40, no. 8, pp. 1381–1391, 2007. View at Publisher · View at Google Scholar · View at Scopus
  16. W. Kopp, T. P. Da Costa, S. C. Pereira et al., “Easily handling penicillin G acylase magnetic cross-linked enzymes aggregates: catalytic and morphological studies,” Journal of Process Biochemistry, vol. 49, no. 1, pp. 38–46, 2014. View at Publisher · View at Google Scholar · View at Scopus
  17. M. G. Antov, “Partitioning of pectinase produced by Polyporus squamosus in aqueous two-phase system polyethylene glycol 4000/crude dextran at different initial pH values,” Carbohydrate Polymers, vol. 56, no. 3, pp. 295–300, 2004. View at Publisher · View at Google Scholar · View at Scopus
  18. P. V. Iyer and L. Ananthanarayan, “Enzyme stability and stabilization—aqueous and non-aqueous environment,” Process Biochemistry, vol. 43, no. 10, pp. 1019–1032, 2008. View at Publisher · View at Google Scholar · View at Scopus
  19. H. U. Hebbar, B. Sumana, and K. S. M. S. Raghavarao, “Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes,” Bioresource Technology, vol. 99, no. 11, pp. 4896–4902, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. J. Y. Imm and S. C. Kim, “Convenient partial purification of polyphenol oxidase from apple skin by cationic reversed micellar extraction,” Food Chemistry, vol. 113, no. 1, pp. 302–306, 2009. View at Publisher · View at Google Scholar · View at Scopus
  21. D. Das and P. K. Das, “Superior activity of structurally deprived enzyme-carbon nanotube hybrids in cationic reverse micelles,” Langmuir, vol. 25, no. 8, pp. 4421–4428, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. C. M. L. Carvalho and J. M. S. Cabral, “Reverse micelles as reaction media for lipases,” Biochimie, vol. 82, no. 11, pp. 1063–1085, 2000. View at Publisher · View at Google Scholar · View at Scopus
  23. J. G. Liu, J. M. Xing, R. Shen, C.-L. Yang, and H.-Z. Liu, “Reverse micelles extraction of nattokinase from fermentation broth,” Biochemical Engineering Journal, vol. 21, no. 3, pp. 273–278, 2004. View at Publisher · View at Google Scholar · View at Scopus
  24. N. Streitner, C. Voß, and E. Flaschel, “Reverse micellar extraction systems for the purification of pharmaceutical grade plasmid DNA,” Journal of Biotechnology, vol. 131, no. 2, pp. 188–196, 2007. View at Publisher · View at Google Scholar · View at Scopus
  25. E. A. Ermakova, N. L. Zakhartchenko, and Y. F. Zuev, “Effect of surface potential of reverse micelle on enzyme-substrate complex formation,” Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 317, no. 1–3, pp. 297–302, 2008. View at Publisher · View at Google Scholar · View at Scopus
  26. H. Zhong, G. M. Zeng, X. Z. Yuan, H. Y. Fu, G. H. Huang, and F. Y. Ren, “Adsorption of dirhamnolipid on four microorganisms and the effect on cell surface hydrophobicity,” Applied Microbiology and Biotechnology, vol. 77, no. 2, pp. 447–455, 2007. View at Publisher · View at Google Scholar · View at Scopus
  27. M. A. Biasutti, E. B. Abuin, J. J. Silber, N. M. Correa, and E. A. Lissi, “Kinetics of reactions catalyzed by enzymes in solutions of surfactants,” Advances in Colloid and Interface Science, vol. 136, no. 1-2, pp. 1–24, 2008. View at Publisher · View at Google Scholar · View at Scopus
  28. U. Sivars and F. Tjerneld, “Mechanisms of phase behaviour and protein partitioning in detergent/polymer aqueous two-phase systems for purification of integral membrane proteins,” Biochimica et Biophysica Acta—General Subjects, vol. 1474, no. 2, pp. 133–146, 2000. View at Publisher · View at Google Scholar · View at Scopus
  29. W. W. Wang, X. Z. Yuan, G. M. Zeng, Y. S. Liang, and Y. Chao, “Enzymatic hydrolysis of cellulose in reverse micelles,” Journal of Environmental Science, vol. 31, no. 9, pp. 2202–2207, 2010. View at Google Scholar · View at Scopus
  30. G. H. Ma, P. Wang, and Z. G. Su, “Nanoscience and enzyme,” Journal of Chinese Basic Science, vol. 5, no. 1, pp. 49–54, 2009. View at Google Scholar
  31. R. C. F. Bonomo, L. A. Minim, J. S. R. Coimbra, R. C. I. Fontan, L. H. M. da Silva, and V. P. R. Minim, “Hydrophobic interaction adsorption of whey proteins: effect of temperature and salt concentration and thermodynamic analysis,” Journal of Chromatography B, vol. 844, no. 1, pp. 6–14, 2006. View at Publisher · View at Google Scholar · View at Scopus
  32. H. S. Mohammadi and E. Omidinia, “Process integration for the recovery and purification of recombinant Pseudomonas fluorescens proline dehydrogenase using aqueous two-phase systems,” Journal of Chromatography B, vol. 929, pp. 11–17, 2013. View at Publisher · View at Google Scholar · View at Scopus
  33. A. Paulus, C. Morhardt, N. Lehle, and M. Franzreb, “Recovery of chymotrypsin using magnetic particles and aqueous micellar two-phase systems: influence of non-ionic surfactants on enzyme activity,” Journal of Molecular Catalysis B: Enzymatic, vol. 110, no. 7, pp. 165–170, 2014. View at Publisher · View at Google Scholar