A Kinase Anchoring Protein 9 Is a Novel Myosin VI Binding Partner That Links Myosin VI with the PKA Pathway in Myogenic Cells

Figure 2

AKAP9 expression in myogenic cells probed by immunodetection. (a) AKAP9 was found in the pull-downs of the day 0, day 3, and day 7 myoblast samples. (b) AKAP9 expression is decreasing during myoblast differentiation into myotubes. Right panel: quantitative analysis of the AKAP9 and MVI content with respect to the level of γ-actin. (c) The level of AKAP9 expression is substantially elevated in MVI-KD cells when compared with untreated C2C12 and scrambled myoblasts. Right panels: quantitative analyses of the AKAP9 and MVI content with respect to the level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The results in (b) and (c) are presented as means ± SD from two experiments; .

(a)

(b)

(c)