Bioprecipitation of Calcium Carbonate Crystals by Bacteria Isolated from Saline Environments Grown in Culture Media Amended with Seawater and Real Brine

Table 4

Carbonate mineral precipitation in solid media and carbonic anhydrase activity.

Strain	Number of days strains take to precipitate						Carbonic anhydrase
	Culture media tested
	SM1	SM2	SM3	SM4	SM5	SM6
Bacillus marisflavi S1	4	6	NF	8	14	14	NR
Bacillus marisflavi S2	4	6	NF	10	NF	NF	NR
Virgibacillus pantothenticus S3	2	2	8	8	14	14	NR
Bacillus pumilus M1	6	8	NF	8	NF	NF	PR
Bacillus pumilus M2	6	10	8	10	16	14	PR
Bacillus marisflavi M3	2	2	8	8	14	14	PR

PR: positive reaction; NR: negative reaction (no yellow colouration). NF: non-crystal-formation.