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BioMed Research International
Volume 2015, Article ID 823806, 8 pages
http://dx.doi.org/10.1155/2015/823806
Research Article

Reference Gene Selection for qPCR Normalization of Kosteletzkya virginica under Salt Stress

1Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai 264003, China
2University of Chinese Academy of Sciences, Beijing 100039, China
3Yantai Academy of China Agricultural University, Yantai 264670, China
4College of Life Sciences, Shandong Agricultural University, Taian 271018, China
5Institute of Agro-Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received 23 June 2015; Revised 9 August 2015; Accepted 11 August 2015

Academic Editor: Raul A. Sperotto

Copyright © 2015 Xiaoli Tang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Kosteletzkya virginica (L.) is a newly introduced perennial halophytic plant. Presently, reverse transcription quantitative real-time PCR (qPCR) is regarded as the best choice for analyzing gene expression and its accuracy mainly depends on the reference genes which are used for gene expression normalization. In this study, we employed qPCR to select the most stable reference gene in K. virginica which showed stable expression profiles under our experimental conditions. The candidate reference genes were 18S ribosomal RNA (18SrRNA), β-actin (ACT), α-tubulin (TUA), and elongation factor (EF). We tracked the gene expression profiles of the candidate genes and analyzed their stabilities through BestKeeper, geNorm, and NormFinder software programs. The results of the three programs were identical and 18SrRNA was assessed to be the most stable reference gene in this study. However, TUA was identified to be the most unstable. Our study proved again that the traditional reference genes indeed displayed a certain degree of variations under given experimental conditions. Importantly, our research also provides guidance for selecting most suitable reference genes and lays the foundation for further studies in K. virginica.