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BioMed Research International
Volume 2015 (2015), Article ID 825468, 9 pages
http://dx.doi.org/10.1155/2015/825468
Research Article

Mosaicism of Mitochondrial Genetic Variation in Atherosclerotic Lesions of the Human Aorta

1Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, 8 Baltiyskaya Street, Moscow 125315, Russia
2Laboratory of Medical Genetics, Russian Cardiology Research and Production Complex, 15a 3rd Cherepkovskaya Street, Moscow 121552, Russia
3Faculty of Medicine, School of Medical Sciences, University of New South Wales, Sydney, Kensington, NSW 2052, Australia
4School of Medicine, University of Western Sydney, Campbelltown, NSW 2560, Australia
5Institute for Atherosclerosis Research, Skolkovo Innovation Centre, Moscow 121552, Russia

Received 25 July 2014; Revised 18 September 2014; Accepted 28 October 2014

Academic Editor: María E. Esteban

Copyright © 2015 Margarita A. Sazonova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. The aim of the present study was an analysis of heteroplasmy level in mitochondrial mutations 652delG, A1555G, C3256T, T3336C, 652insG, C5178A, G12315A, G13513A, G14459A, G14846A, and G15059A in normal and affected by atherosclerosis segments of morphologically mapped aortic walls. Methods. We investigated the 265 normal and atherosclerotic tissue sections of 5 human aortas. Intima of every aorta was divided according to morphological characteristics into segments with different types of atherosclerotic lesions: fibrous plaque, lipofibrous plaque, primary atherosclerotic lesion (fatty streak and fatty infiltration), and normal intima from human aorta. PCR-fragments were analyzed by a new original method developed in our laboratory on the basis of pyrosequence technology. Results. According to the obtained data, mutations G12315A and G14459A are significantly associated with total and primary atherosclerotic lesions of intimal segments and lipofibrous plaques ( and , accordingly). Mutation C5178A is significantly associated with fibrous plaques and total atherosclerotic lesions . A1555G mutation shows an antiatherosclerotic effect in primary lesion in lipofibrous plaques . Meanwhile, G14846A mutation is antiatherogenic for lipofibrous plaques . Conclusion. Therefore, mutations C5178A, G14459A, G12315A, A1555G, and G14846A were found to be associated with atherosclerotic lesions.