Cosuppression of Sprouty and Sprouty-Related Negative Regulators of FGF Signalling in Prostate Cancer: A Working Hypothesis

Figure 4

Immunocytochemical analysis of proliferating cell nuclear antigen (PCNA) protein in prostates of hemizygous and homozygous null Spry mice. Representative 5 μm thin sections of (a) prostate, whole serum negative control; (b) prostate, no primary antibody negative control; (c) positive control of mouse testis showing immunopositive (brown) proliferating spermatogonia but immunonegative haematoxylin counterstained (blue) developing spermatids; immunopositive nuclei in (d) Spry1^+/−, (e) Spry1^−/−, (f) Spry2^+/−, and (g) Spry2^−/− prostates. Scale bar = 25 μm.

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