Research Article

How to Quantify Penile Corpus Cavernosum Structures with Histomorphometry: Comparison of Two Methods

Table 1

Smooth muscle surface density of corpus cavernosum of Wistar Kyoto normotensive animals (WKY) and spontaneously hypertensive rats (H) as measured by two different morphometrical methods.

ObserverWKYHP value

A
 Point-counting method (%)10.30 3.0813.28 1.330.012
 Color-based segmentation method (%)09.18 2.7211.48 1.760.038

P value 0.1190.001

B
 Point-counting method (%)11.08 2.2713.94 1.450.003
 Color-based segmentation method (%)07.85 0.9310.83 2.340.002

P value 0.0010.002

Data are shown as mean standard deviation. Means were considered significantly different if P < 0.05.