Research Article

POD-1/TCF21 Reduces SHP Expression, Affecting LRH-1 Regulation and Cell Cycle Balance in Adrenocortical and Hepatocarcinoma Tumor Cells

Figure 2

Quantitative reverse transcription RT-PCR (qRT-PCR) and immunoblotting analysis of relative SHP/SHP expression. Relative POD-1/β-Actin mRNA levels in HepG2 cells transiently transfected with the empty vector pCMVMyc or with pCMVMycPod-1 (a); SHP/β-Actin mRNA levels in H295R cells transiently transfected with the empty vector pCMVMyc or with pCMVMycPod-1 (b); SHP/β-Actin mRNA levels and SHP/α-actinin protein levels in HepG2 cells (c and d, resp.). Total RNA samples and protein samples were prepared 48 h and 72 h after transfection, respectively. Differences were tested with paired samples -tests. Values represent means ± standard deviations from 3 experiments.
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