POD-1/TCF21 Reduces SHP Expression, Affecting LRH-1 Regulation and Cell Cycle Balance in Adrenocortical and Hepatocarcinoma Tumor Cells

Figure 3

Chromatin enrichment was confirmed by PCR amplification of the E-box region of the SHP-1 promoter from anti-MYC immunoprecipitated HepG2pCMVMycPod-1 DNA. The positions of the amplicons in relation to the Transcriptional Start Sites (TSSs) (represented by arrows) are shown. Black and open bars represent the exon and a different E-box sequence, respectively. Androgen receptor (AR), input (Inp) 0.1% DNA, Anti-MYC-IP HepG2pCMVMycPod-1 (IP), and anti-IgG (IgG).