Tyrosine 705 Phosphorylation of STAT3 Is Associated with Phenotype Severity in TGFβ1 Transgenic Mice

Figure 3

STAT3 phosphorylation level in differentiated C2C12. The panel shows STAT3 phosphorylation in differentiated C2C12 after TGFβ1 10 ng/mL treatment. Protein extracted from C2C12 in control conditions, after 30 minutes, 2 hours, and 24 hours after TGFβ1 10 ng/mL treatment, with anti-phospho-STAT3 (Tyr705), anti-total STAT3, and anti-GAPDH. The graph shows fold changes of phospho-STAT3 (Tyr705) normalized to GAPDH ± SEM. indicates ; indicates .