The Use and Effectiveness of Triple Multiplex System for Coding Region Single Nucleotide Polymorphism in Mitochondrial DNA Typing of Archaeologically Obtained Human Skeletons from Premodern Joseon Tombs of Korea

Figure 2

Analysis on sample number 038. (a) SNP analysis of mtDNA coding region by a SNaPshot Kit. ((b), (c)) Direct sequencing analysis on mtDNA control region. (b) Electrophoresis of PCR amplicons. (c) Direct sequencing result. rCRS, revised Cambridge Reference Sequence. Although mtDNA haplogroup expected by coding region SNP analysis could fall successfully within the same haplogroup that control region sequencing could make, haplogroup subclade made by SNP analysis (D4b) was not as successful as seen in control region sequencing (D4b2b).

(a) Coding region

(b) Control region

(c) Sequencing result of control region