Research Article

The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the Bladder

Figure 2

Macrophages and MIF expression in the urothelium from the rats at 1, 2, and 4 weeks after implantation. The control, sham-operated (a), PDMS- (b), and PMMA- (c) coated metal were shown. The figure shows macrophages immunostaining (red immunofluorescence), MIF immunostaining (green immunofluorescence), and a DAPI nuclear stain (blue immunofluorescence). Macrophages and MIF were not expressed in the control and sham-operated groups (a) at 1, 2, and 4 weeks. Increased expression of macrophages and MIF was observed in the urothelium after PDMS- and PMMA-coated metal implantation at 1 week (b and c). MIF was detected in basal and intermediate layers of the urothelium at 1 week. Macrophages and MIF expression were decreased and only detected in basal layer at 2 weeks and rarely expressed at 4 weeks in the rats implanted with PDMS- and PMMA-coated metal (b and c; arrows). Scale bar = 100 μm.
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