Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Sesame Seed in Foods
Table 2
Recovery of sesame form bread spiked with sesame flour using different ELISA methods.
Bread sample
Spike level
Current method1
Neogen-Tepnel2
ELISA Systems3
Sesame (ppm)
Recovery (%)
Sesame (ppm)
Recovery (%)
Sesame (ppm)
Recovery (%)
Middle part
0
—
n.a.
—
n.a.
—
n.a.
1
—
n.a.
0.34
34
0.16
16
5
0.43
9
1.45
29
0.85
17
10
0.61
6
4.56
46
1.15
12
50
2.28
5
24.3
49
6.5
13
100
6.53
7
21.8
22
14.4
14
Lighter crust end
0
BLQ
n.a.
—
n.a.
—
n.a.
1
0.18
18
0.95
95
0.18
18
5
0.49
10
2.6
52
0.63
13
10
0.49
5
4.3
43
1.18
12
50
2.97
6
17.5
35
5.73
11
100
7.29
7
42.3
42
13.3
13
Darker crust end
0
—
n.a.
—
n.a.
—
n.a.
1
—
n.a.
0.41
41
0.14
14
5
0.17
3
0.61
12
0.6
12
10
0.31
3
2.8
28
1.09
11
50
1.55
3
16.6
33
5.62
11
100
2.05
2
16.6
17
12
12
1: defined LLOQ is 0.5 ppm. Values between 0.1 and 0.5 ppm may be therefore imprecise. 2: LLOQ given by manufacturer is 6 ppm. Values below 6 ppm may be therefore imprecise. 3: LLOQ given by manufacturer is 0.5 ppm. Values below 0.5 ppm may be therefore imprecise. n.a.: not applicable.
