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BioMed Research International
Volume 2015 (2015), Article ID 854078, 6 pages
http://dx.doi.org/10.1155/2015/854078
Research Article

Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens

1Infectious Diseases and Microbiology Service, Regional University and Virgen de la Victoria University Hospitals, IBIMA, 29010 Malaga, Spain
2Microbiology Unit, Hospital Costa del Sol, 29603 Marbella, Spain

Received 25 July 2014; Revised 18 October 2014; Accepted 19 October 2014

Academic Editor: Mehdi Mirsaeidi

Copyright © 2015 María Concepción Mediavilla-Gradolph et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various clinical specimens (mainly respiratory) were tested in this study. They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType Mycobacterium from liquid culture MGIT. Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice (M. avium, M. intracellulare, M. abscessus, M. chelonae, M. fortuitum, M. mucogenicum, M. kansasii, and M. scrofulaceum). MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species.