Effect and Mechanism of Total Flavonoids Extracted from Cotinus coggygria against Glioblastoma Cancer In Vitro and In Vivo

Figure 5

Apoptosis induced by CCF was associated with the PI3K/AKT/ERK signaling pathway. (a) Phosphorylation of Akt (Ser473) was significantly lower in U87, U251, and DBTRG-05MG glioblastoma cells treated with CCF. CCF (100 μg/mL) induced the downregulation of Akt levels in U87, U251, and DBTRG-05MG glioblastoma cells. (b) Compared with the control, CCF had no significant effects on ERK levels, and phospho-ERK levels were downregulated by CCF in U87, U251, and DBTRG-05MG glioblastoma cells. , , and , versus the control.

(a)

(b)