Shear Stress Induces Differentiation of Endothelial Lineage Cells to Protect Neonatal Brain from Hypoxic-Ischemic Injury through NRP1 and VEGFR2 Signaling

Figure 4

Hypoxia promoted the migration ability of cells, especially in ELCs. The transmigration of ASCs and ELCs was measured by in vitro Boyden chamber assay with or without the application of hypoxia mimetic reagent (DFO). The cell mobility was significantly increased in ELCs under the hypoxia (a). The NRP1 and VEGFR2 were increased in ELCs and further enhanced with DFO treatment (b). The phosphorylation of Akt (p-Akt) was increased in ELCs and also enhanced under hypoxia (c). , Scale bar: 200 μm. < 0.05 compared to undifferentiated ASCs under normoxia. < 0.05 compared to undifferentiated ASCs under hypoxia.

(a)

(b)

(c)