Shear Stress Induces Differentiation of Endothelial Lineage Cells to Protect Neonatal Brain from Hypoxic-Ischemic Injury through NRP1 and VEGFR2 Signaling
The enhancement of cell migration under hypoxia in ELCs was inhibited by using specific inhibitors to block the NRP1 (DG2) and VEGFR (BIBF1120) signaling (a). Blockage of VEGFR by BIBF1120 decreased the endothelial gene expressions (b). When treating the hypoxic ELCs with NRP1 inhibitor (DG2), both the p-Akt and total Akt were decreased (c). The treatment of VEGF inhibitors totally abolished the Akt and ERK signals. , Scale bar: 200 μm. < 0.05 compared to ELCs under normoxia. < 0.05 compared to ELC under hypoxia.