Loop Mediated Isothermal Amplification for Detection of Trypanosoma brucei gambiense in Urine and Saliva Samples in Nonhuman Primate Model

Table 3

Comparative analysis of LAMP, PCR, and parasitological techniques in trypanosome detection in serum, saliva, and urine samples obtained from monkeys infected with T. b. gambiense IL3253.

Test	Kappa value	Level of agreement	statistic	value
PCR and parasitology	0.83 (0.36–0.59)	Very good	2.22	0.136
LAMP and parasitology	0.48 (0.36–0.59)	Marginal	26.47	<0.001
LAMP and PCR (serum)	0.59 (0.47–0.72)	Marginal	10.42	0.0012
LAMP and PCR (saliva)	0.14 (0.06–0.21)	Poor	79.79	<0.0001
LAMP and PCR (urine)	0	Poor	6.12	0.0134