Extraction Optimization for Obtaining Artemisia capillaris Extract with High Anti-Inflammatory Activity in RAW 264.7 Macrophage Cells

Table 1

The central composite experimental design and experimental data for the optimization of conditions for obtaining  Artemisia capillaris Thunb. extracts.

Run	Independent variables^1			Response variables^2
	(°C)	(%)	(h)	(%)	(%)	(%)
1	42 (−1)	20 (−1)	3 (−1)	73.38 ± 0.21	79.18 ± 1.19	87.34 ± 2.04
2	78 (1)	20 (−1)	3 (−1)	78.81 ± 0.15	83.32 ± 0.24	84.98 ± 4.38
3	42 (−1)	80 (1)	3 (−1)	86.69 ± 0.13	87.21 ± 0.33	80.36 ± 0.52
4	78 (1)	80 (1)	3 (−1)	67.11 ± 0.23	69.34 ± 0.32	81.46 ± 0.89
5	42 (−1)	20 (−1)	9 (1)	74.38 ± 0.58	69.55 ± 1.06	85.98 ± 1.19
6	78 (1)	20 (−1)	9 (1)	85.69 ± 0.79	85.08 ± 0.88	82.56 ± 1.34
7	42 (−1)	80 (1)	9 (1)	68.32 ± 0.25	69.50 ± 0.24	82.88 ± 1.34
8	78 (1)	80 (1)	9 (1)	73.38 ± 0.14	72.50 ± 0.16	86.92 ± 2.15
9	30 (−1.682)	50 (0)	6 (0)	75.15 ± 0.42	78.66 ± 0.24	89.89 ± 0.41
10	90 (1.682)	50 (0)	6 (0)	63.41 ± 0.11	63.18 ± 0.31	88.13 ± 2.75
11	60 (0)	0 (−1.682)	6 (0)	71.65 ± 0.52	80.68 ± 0.24	85.24 ± 0.37
12	60 (0)	100 (1.682)	6 (0)	75.15 ± 0.81	83.84 ± 0.16	85.50 ± 1.41
13	60 (0)	50 (0)	1 (−1.682)	69.01 ± 0.19	75.92 ± 0.27	80.28 ± 1.30
14	60 (0)	50 (0)	11 (1.682)	66.71 ± 0.14	71.36 ± 0.32	88.00 ± 0.26
15	60 (0)	50 (0)	6 (0)	53.21 ± 0.21	56.50 ± 0.98	90.47 ± 1.26
16	60 (0)	50 (0)	6 (0)	54.14 ± 0.47	56.03 ± 1.79	91.20 ± 1.15
17	60 (0)	50 (0)	6 (0)	54.36 ± 0.23	56.03 ± 0.86	90.75 ± 1.89
18	60 (0)	50 (0)	6 (0)	54.75 ± 0.15	57.12 ± 0.28	90.13 ± 0.74
19	60 (0)	50 (0)	6 (0)	53.97 ± 0.50	56.65 ± 0.91	91.75 ± 1.71
20	60 (0)	50 (0)	6 (0)	54.36 ± 0.14	55.87 ± 1.07	90.13 ± 2.34

Independent variables: , extraction temperature; , ethanol concentration; , extraction time.
²Response variables: , LPS-induced PGE₂ production; , LPS-induced NO production; , cytotoxicity.