Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2015, Article ID 873251, 10 pages
http://dx.doi.org/10.1155/2015/873251
Research Article

Mechanical Strain Regulates Osteogenic and Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

1Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
2Huiqiao Department, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
3Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

Received 28 December 2014; Revised 27 February 2015; Accepted 10 March 2015

Academic Editor: Cijun Shuai

Copyright © 2015 Runguang Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. N. Stefan, H.-U. Häring, F. B. Hu, and M. B. Schulze, “Metabolically healthy obesity: epidemiology, mechanisms, and clinical implications,” The Lancet Diabetes and Endocrinology, vol. 1, no. 2, pp. 152–162, 2013. View at Publisher · View at Google Scholar · View at Scopus
  2. W. D. Leslie and S. N. Morin, “Osteoporosis epidemiology 2013: implications for diagnosis, risk assessment, and treatment,” Current Opinion in Rheumatology, vol. 26, no. 4, pp. 440–446, 2014. View at Publisher · View at Google Scholar · View at Scopus
  3. S. W. Coppack, “Adipose tissue changes in obesity,” Biochemical Society Transactions, vol. 33, no. 5, pp. 1049–1052, 2005. View at Publisher · View at Google Scholar · View at Scopus
  4. M. Kawai, F. J. A. de Paula, and C. J. Rosen, “New insights into osteoporosis: the bone-fat connection,” Journal of Internal Medicine, vol. 272, no. 4, pp. 317–329, 2012. View at Publisher · View at Google Scholar · View at Scopus
  5. M. F. Pittenger, A. M. Mackay, S. C. Beck et al., “Multilineage potential of adult human mesenchymal stem cells,” Science, vol. 284, no. 5411, pp. 143–147, 1999. View at Publisher · View at Google Scholar · View at Scopus
  6. Z.-J. Liu, Y. Zhuge, and O. C. Velazquez, “Trafficking and differentiation of mesenchymal stem cells,” Journal of Cellular Biochemistry, vol. 106, no. 6, pp. 984–991, 2009. View at Publisher · View at Google Scholar · View at Scopus
  7. M. L. Knothe Tate, T. D. Falls, S. H. McBride, R. Atit, and U. R. Knothe, “Mechanical modulation of osteochondroprogenitor cell fate,” International Journal of Biochemistry & Cell Biology, vol. 40, no. 12, pp. 2720–2738, 2008. View at Publisher · View at Google Scholar · View at Scopus
  8. L. Zhao and B. M. Hantash, “TGF-β1 regulates differentiation of bone marrow mesenchymal stem cells,” Vitamins and Hormones, vol. 87, pp. 127–141, 2011. View at Publisher · View at Google Scholar · View at Scopus
  9. T. J. Heino and T. A. Hentunen, “Differentiation of osteblasts and osteocytes from mesenchymal stem cells,” Current Stem Cell Research & Therapy, vol. 3, no. 2, pp. 131–145, 2008. View at Publisher · View at Google Scholar · View at Scopus
  10. J.-H. Hong, E. S. Hwang, M. T. McManus et al., “TAZ, a transcriptional modulator of mesenchymal stem cell differentiation,” Science, vol. 309, no. 5737, pp. 1074–1078, 2005. View at Publisher · View at Google Scholar · View at Scopus
  11. S. Zhou, K. Eid, and J. Glowacki, “Cooperation between TGF-beta and Wnt pathways during chondrocyte and adipocyte differentiation of human marrow stromal cells,” Journal of Bone and Mineral Research, vol. 19, no. 3, pp. 463–470, 2004. View at Publisher · View at Google Scholar · View at Scopus
  12. M. A. Adams, “The mechanical environment of chondrocytes in articular cartilage,” Biorheology, vol. 43, no. 3-4, pp. 537–545, 2006. View at Google Scholar · View at Scopus
  13. J. S. Park, N. F. Huang, K. T. Kurpinski, S. Patel, S. Hsu, and S. Li, “Mechanobiology of mesenchymal stem cells and their use in cardiovascular repair,” Frontiers in Bioscience, vol. 12, no. 13, pp. 5098–5116, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. G. Z. Genchev, M. Källberg, G. Gürsoy et al., “Mechanical signaling on the single protein level studied using steered molecular dynamics,” Cell Biochemistry and Biophysics, vol. 55, no. 3, pp. 141–152, 2009. View at Publisher · View at Google Scholar · View at Scopus
  15. H. M. Frost, “A 2003 update of bone physiology and Wolff s law for clinicians,” Angle Orthodontist, vol. 74, no. 1, pp. 3–15, 2004. View at Google Scholar · View at Scopus
  16. M.-H. Lafage-Proust, P. Collet, J. M. Dubost, N. Laroche, C. Alexandre, and L. Vico, “Space-related bone mineral redistribution and lack of bone mass recovery after reambulation in young rats,” The American Journal of Physiology—Regulatory Integrative and Comparative Physiology, vol. 274, no. 2, pp. R324–R334, 1998. View at Google Scholar · View at Scopus
  17. W. Dehority, B. P. Halloran, D. D. Bikle et al., “Bone and hormonal changes induced by skeletal unloading in the mature male rat,” The American Journal of Physiolog—Endocrinology and Metabolism, vol. 276, no. 1, pp. E62–E69, 1999. View at Google Scholar · View at Scopus
  18. Y. Bei, X. Fang, and Z. Yao, “Sixty-two cases of simple obesity treated by acupuncture combined with massage,” Journal of Traditional Chinese Medicine, vol. 24, no. 1, pp. 36–39, 2004. View at Google Scholar · View at Scopus
  19. C. T. Rubin, E. Capilla, Y. K. Luu et al., “Adipogenesis is inhibited by brief, daily exposure to high-frequency, extremely low-magnitude mechanical signals,” Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 45, pp. 17879–17884, 2007. View at Publisher · View at Google Scholar · View at Scopus
  20. X. Zeng, S. P. Yu, T. Taylor, M. Ogle, and L. Wei, “Protective effect of apelin on cultured rat bone marrow mesenchymal stem cells against apoptosis,” Stem Cell Research, vol. 8, no. 3, pp. 357–367, 2012. View at Publisher · View at Google Scholar · View at Scopus
  21. N. J. Turner, H. S. Jones, J. E. Davies, and A. E. Canfield, “Cyclic stretch-induced TGFβ1/Smad signaling inhibits adipogenesis in umbilical cord progenitor cells,” Biochemical and Biophysical Research Communications, vol. 377, no. 4, pp. 1147–1151, 2008. View at Publisher · View at Google Scholar · View at Scopus
  22. B. Lohberger, H. Kaltenegger, N. Stuendl, M. Payer, B. Rinner, and A. Leithner, “Effect of cyclic mechanical stimulation on the expression of osteogenesis genes in human intraoral mesenchymal stromal and progenitor cells,” BioMed Research International, vol. 2014, Article ID 189516, 10 pages, 2014. View at Publisher · View at Google Scholar · View at Scopus
  23. G. Khayat, D. H. Rosenzweig, and T. M. Quinn, “Low frequency mechanical stimulation inhibits adipogenic differentiation of C3H10T1/2 mesenchymal stem cells,” Differentiation, vol. 83, no. 4, pp. 179–184, 2012. View at Publisher · View at Google Scholar · View at Scopus
  24. B. Sen, Z. Xie, N. Case, M. Ma, C. Rubin, and J. Rubin, “Mechanical strain inhibits adipogenesis in mesenchymal stem cells by stimulating a durable beta-catenin signal,” Endocrinology, vol. 149, no. 12, pp. 6065–6075, 2008. View at Publisher · View at Google Scholar · View at Scopus
  25. Y. P. Sun, B. L. Zhang, J. W. Duan et al., “Effect of NK4 transduction in bone marrow-derived mesenchymal stem cells on biological characteristics of pancreatic cancer cells,” International Journal of Molecular Sciences, vol. 15, pp. 3729–3745, 2014. View at Publisher · View at Google Scholar
  26. N. Liu, R. Chen, H. Du, J. Wang, Y. Zhang, and J. Wen, “Expression of IL-10 and TNF-alpha in rats with cerebral infarction after transplantation with mesenchymal stem cells,” Cellular & Molecular Immunology, vol. 6, no. 3, pp. 207–213, 2009. View at Publisher · View at Google Scholar · View at Scopus
  27. D. E. Jaalouk and J. Lammerding, “Mechanotransduction gone awry,” Nature Reviews Molecular Cell Biology, vol. 10, no. 1, pp. 63–73, 2009. View at Publisher · View at Google Scholar · View at Scopus
  28. K. T. S. Palomares, R. E. Gleason, Z. D. Mason et al., “Mechanical stimulation alters tissue differentiation and molecular expression during bone healing,” Journal of Orthopaedic Research, vol. 27, no. 9, pp. 1123–1132, 2009. View at Publisher · View at Google Scholar · View at Scopus
  29. J. D. Boerckel, K. M. Dupont, Y. M. Kolambkar, A. S. P. Lin, and R. E. Guldberg, “In vivo model for evaluating the effects of mechanical stimulation on tissue-engineered bone repair,” Journal of Biomechanical Engineering, vol. 131, no. 8, Article ID 084502-1, 2009. View at Publisher · View at Google Scholar · View at Scopus
  30. S. R. Park, R. O. C. Oreffo, and J. T. Triffitt, “Interconversion potential of cloned human marrow adipocytes in vitro,” Bone, vol. 24, no. 6, pp. 549–554, 1999. View at Publisher · View at Google Scholar · View at Scopus
  31. P. J. Marie, “Transcription factors controlling osteoblastogenesis,” Archives of Biochemistry and Biophysics, vol. 473, no. 2, pp. 98–105, 2008. View at Publisher · View at Google Scholar · View at Scopus
  32. K. Nakashima, X. Zhou, G. Kunkel et al., “The novel zinc finger-containing transcription factor Osterix is required for osteoblast differentiation and bone formation,” Cell, vol. 108, no. 1, pp. 17–29, 2002. View at Publisher · View at Google Scholar · View at Scopus
  33. W. Pradel, R. Mai, T. Gedrange, and G. Lauer, “Cell passage and composition of culture medium effects proliferation and differentiation of human osteoblast-like cells from facial bone,” Journal of Physiology and Pharmacology, vol. 59, supplement 5, pp. 47–58, 2008. View at Google Scholar · View at Scopus
  34. C. A. Simmons, S. Matlis, A. J. Thornton, S. Chen, C.-Y. Wang, and D. J. Mooney, “Cyclic strain enhances matrix mineralization by adult human mesenchymal stem cells via the extracellular signal-regulated kinase (ERK1/2) signaling pathway,” Journal of Biomechanics, vol. 36, no. 8, pp. 1087–1096, 2003. View at Publisher · View at Google Scholar · View at Scopus
  35. A. G. Cristancho and M. A. Lazar, “Forming functional fat: a growing understanding of adipocyte differentiation,” Nature Reviews Molecular Cell Biology, vol. 12, no. 11, pp. 722–734, 2011. View at Publisher · View at Google Scholar · View at Scopus
  36. E. D. Rosen and O. A. MacDougald, “Adipocyte differentiation from the inside out,” Nature Reviews Molecular Cell Biology, vol. 7, no. 12, pp. 885–896, 2006. View at Publisher · View at Google Scholar · View at Scopus
  37. H. G. Linhart, K. Ishimura-Oka, F. Demayo et al., “C/EBPalpha is required for differentiation of white, but not brown, adipose tissue,” Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 22, pp. 12532–12537, 2001. View at Publisher · View at Google Scholar · View at Scopus
  38. J. Zhang, M. Fu, T. Cui et al., “Selective disruption of PPARγ2 impairs the development of adipose tissue and insulin sensitivity,” Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 29, pp. 10703–10708, 2004. View at Publisher · View at Google Scholar · View at Scopus
  39. N. Case, J. Thomas, Z. Xie et al., “Mechanical input restrains PPARgamma2 expression and action to preserve mesenchymal stem cell multipotentiality,” Bone, vol. 52, no. 1, pp. 454–464, 2013. View at Publisher · View at Google Scholar · View at Scopus
  40. A. Charoenpanich, M. E. Wall, C. J. Tucker et al., “Cyclic tensile strain enhances osteogenesis and angiogenesis in mesenchymal stem cells from osteoporotic donors,” Tissue Engineering Part A, vol. 20, no. 1-2, pp. 67–78, 2014. View at Publisher · View at Google Scholar · View at Scopus
  41. D. F. Ward Jr., R. M. Salasznyk, R. F. Klees et al., “Mechanical strain enhances extracellular matrix-induced gene focusing and promotes osteogenic differentiation of human mesenchymal stem cells through an extracellular-related kinase-dependent pathway,” Stem Cells and Development, vol. 16, no. 3, pp. 467–479, 2007. View at Publisher · View at Google Scholar · View at Scopus
  42. E. M. Kearney, E. Farrell, P. J. Prendergast, and V. A. Campbell, “Tensile strain as a regulator of mesenchymal stem cell osteogenesis,” Annals of Biomedical Engineering, vol. 38, no. 5, pp. 1767–1779, 2010. View at Publisher · View at Google Scholar · View at Scopus
  43. R. G. Li, B. Chen, G. Wang, B. Yu, G. H. Ren, and G. X. Ni, “Effects of mechanical strain on oxygen free radical system in bone marrow mesenchymal stem cells from children,” Injury, vol. 42, no. 8, pp. 753–757, 2011. View at Publisher · View at Google Scholar · View at Scopus
  44. J. Klein-Nulend, A. D. Bakker, R. G. Bacabac, A. Vatsa, and S. Weinbaum, “Mechanosensation and transduction in osteocytes,” Bone, vol. 54, no. 2, pp. 182–190, 2013. View at Publisher · View at Google Scholar · View at Scopus
  45. E. Potier, J. Noailly, and K. Ito, “Directing bone marrow-derived stromal cell function with mechanics,” Journal of Biomechanics, vol. 43, no. 5, pp. 807–817, 2010. View at Publisher · View at Google Scholar · View at Scopus
  46. N. Shoham and A. Gefen, “Mechanotransduction in adipocytes,” Journal of Biomechanics, vol. 45, no. 1, pp. 1–8, 2012. View at Publisher · View at Google Scholar · View at Scopus
  47. R. Li, M. Wei, and J. Shao, “Effects of verapamil on the immediate-early gene expression of bone marrow mesenchymal stem cells stimulated by mechanical strain in vitro,” Medical Science Monitor Basic Research, vol. 19, pp. 68–75, 2013. View at Publisher · View at Google Scholar · View at Scopus
  48. Y. Tanabe, Y. Matsunaga, M. Saito, and K. Nakayama, “Involvement of cyclooxygenase-2 in synergistic effect of cyclic stretching and eicosapentaenoic acid on adipocyte differentiation,” Journal of Pharmacological Sciences, vol. 106, no. 3, pp. 478–484, 2008. View at Publisher · View at Google Scholar · View at Scopus
  49. Y. Tanabe, M. Koga, M. Saito, Y. Matsunaga, and K. Nakayama, “Inhibition of adipocyte differentiation by mechanical stretching through ERK-mediated downregulation of PPARgamma2,” Journal of Cell Science, vol. 117, no. 16, pp. 3605–3614, 2004. View at Publisher · View at Google Scholar · View at Scopus
  50. M. G. Hossain, T. Iwata, N. Mizusawa et al., “Compressive force inhibits adipogenesis through COX-2-mediated down-regulation of PPARgamma2 and C/EBPalpha,” Journal of Bioscience and Bioengineering, vol. 109, no. 3, pp. 297–303, 2010. View at Publisher · View at Google Scholar · View at Scopus
  51. M. Zayzafoon, W. E. Gathings, and J. M. McDonald, “Modeled microgravity inhibits osteogenic differentiation of human mesenchymal stem cells and increases adipogenesis,” Endocrinology, vol. 145, no. 5, pp. 2421–2432, 2004. View at Publisher · View at Google Scholar · View at Scopus