Putative Epimutagens in Maternal Peripheral and Cord Blood Samples Identified Using Human Induced Pluripotent Stem Cells

Table 2

PCR primers used.

(a)

Bisulfite PCR primers	Primers (5′ to 3′)	Size (bp)	From transcription start site
SUSD1	Forward: TGGGGTTTATGAGGGTAAGGT	214	1.5 kbp downstream
	Reverse: CCACACCACACACAACCAAT
BRD1	Forward: GGTTTAGGTGTTTGAAGATTTGGT	378	500 bp upstream
	Reverse: ATAAATACCCCTAATCCCCCTAAA
NEBL	Forward: ATTTGGAAATAGGGAGGAGTAATTTT	262	1.5 kbp upstream
	Reverse: TCTCAACAACTTATTTTCTTACAACACA
GLI3	Forward: TGTGGTTTATGTTTGGAATTG	183	2.0 kbp downstream
	Reverse: TCACTAACTCTTCACCCACAATTTA
POU2F1	Forward: TTTAAATTATTTTGTTTTGGGGATG	490	2.5 kbp downstream
	Reverse: TCTACCTCTCACAAACCAACTATCC
HOXB6	Forward: TTTTATGTGGGGTTTAGTAGTTTGG	269	1.5 kbp upstream
	Reverse: ACACATTCACACTCACAAACACATTA
HOXA3	Forward: TGAAAGGGAAGGGGTTGTTT	216	1.5 kbp downstream
	Reverse: TCCCTATATTATACACTATCCCAAAAA
CBX8	Forward: TGGGTTTGTTATTTATTTTGTTGGTA	357	1.0 kbp downstream
	Reverse: CTACCCCACTCTTAAAACCATCTTCT
NANOG	Forward: TTATGGGTTTAGGTATGGTGGAAATA	291	500 bp downstream
	Reverse: AAAACTACCCAATAACATCCACAAAC
BMP2	Forward: ATAGTTTTGGGAAAGTAGAATTTGGT	379	1.5 kbp upstream
	Reverse: TATTTATCTCACCCAACTCAAAAACA

(b)

RT-PCR primers	Primers (5′ to 3′)	Size (bp)
MAP2	Forward: CAGGTGGCGGACGTGTGAAAATTGAGAGTG	212
	Reverse: CACGCTGGATCTGCCTGGGGACTGTG
PAX6	Forward: ACCCATTATCCAGATGTGTTTGCCCGAG	317
	Reverse: ATGGTGAAGCTGGGCATAGGCGGCAG
NES	Forward: CTCCAAGACTTCCCTCAGCTTT	163
	Reverse: CTTAAGAAAGGCTGGCACAGGT
GAPDH	Forward: CAAGATCAGCAATGCCT	68
	Reverse: CTTCCACGATACCAAAGTTGTC