Dissecting the Role of Curcumin in Tumour Growth and Angiogenesis in Mouse Model of Human Breast Cancer

Figure 3

Curcumin inhibits NF-κB activation and downregulates NF-κB-regulated gene products in breast tumors. (a) EMSA assay performed on tumor tissue samples showed the inhibition of NF-κB by curcumin. (b) Immunohistochemistry for PECAM-1 showed the inhibition of PECAM-1 expression in curcumin-treated group, compared to controls. (c) Western blot showing that curcumin inhibits the expression of NF-κB–dependent gene products, cyclin D1, and p-65 in breast tumor tissues. Samples from three animals in each group were analyzed and representative data are shown. β-tubulin was used as loading control.

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