BioMed Research International / 2015 / Article / Fig 2

Research Article

A New Methodology for Evaluation of Nematode Viability

Figure 2

Analysis of viability using fluorimetric markers. (a) Microplate with serial dilution of C. elegans L3 (first point 2,000 to 62). Rows A–D dead larvae; E–H represent viable (control) larvae. Correlation between the number of dead larvae with 50% methanol and the fluorescence intensity of the pixels with Sytox (b) and propidium Iodide (c). Comparison of densitometry (number of pixels) between Sytox and propidium iodide markers (d). Significant differences () were only detected when 1000 and 500 larvae were tested.

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.