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BioMed Research International
Volume 2015 (2015), Article ID 879263, 7 pages
http://dx.doi.org/10.1155/2015/879263
Research Article

A New Methodology for Evaluation of Nematode Viability

1Departamento de Parasitologia, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil
2Departamento de Medicina Veterinária, Universidade Federal de Viçosa, Avenida P.H. Rolfs, s/n, 36570-000 Viçosa, MG, Brazil

Received 18 November 2014; Accepted 19 February 2015

Academic Editor: Ernesto S. Nakayasu

Copyright © 2015 Sebastião Rodrigo Ferreira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The Supplementary Material demonstrate that fluorimetric markers employed in the study does not stain viable live larvae (Supplementary Figure 1) and absence of autofluorescence emitted by the larvae, demonstrate by the lack of fluorescence when dead larvae (treated with methanol) were not stained with Sytox and Propidium Iodide. Os marcadores utilizados não foram eficazes em marcar larvas viáveis (não tratadas). A fluorescência analisada referente às larvas não tratadas com metanol não apresentava proporcionalidade, de acordo os parâmetros estabelecidos.

  1. Supplementary Material