T Lymphocyte Inhibition by Tumor-Infiltrating Dendritic Cells Involves Ectonucleotidase CD39 but Not Arginase-1

Figure 2

TIDC are immunosuppressive. (a) Autologous T-cell proliferation was measured after 5 days of culture with anti-CD3/anti-CD28-coated beads (T(st)) in the presence of spDC or TIDC isolated from 4T1 or LLC tumors at the indicated ratios. Data are representative of 5 independent experiments. (b) CD25 expression by T cells was measured after 5 days of culture with TIDC or spDC. Data are representative of 5 independent experiments. (c) IL-12p70 concentration was quantified by ELISA in TIDC isolated from 4T1 or BMDC 24 hr culture supernatant. (d) IFN-γ concentration was quantified in the supernatant of stimulated T cells cultured alone or in the presence of spDC or TIDC for 5 days.

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