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BioMed Research International
Volume 2015 (2015), Article ID 892013, 12 pages
http://dx.doi.org/10.1155/2015/892013
Research Article

454-Pyrosequencing Analysis of Bacterial Communities from Autotrophic Nitrogen Removal Bioreactors Utilizing Universal Primers: Effect of Annealing Temperature

1Department of Civil Engineering, University of Granada, Campus de Fuentenueva, s/n, 18071 Granada, Spain
2Institute of Water Research, University of Granada, C/Ramón y Cajal 4, 18071 Granada, Spain
3Department of Biotechnology, Technical University of Delft, Julianalaan 67, 2628 BC Delft, Netherlands

Received 20 October 2014; Accepted 26 February 2015

Academic Editor: Weixing Feng

Copyright © 2015 Alejandro Gonzalez-Martinez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table S1 shows main operational parameters of the five bioreactors analyzed in this study.

Table S2 offers the results of the in silico primer testing using RDP database for all bacterial phyla.

Table S3 shows the in silico calculated coverage of anammox bacteria species by primer 530F using the RDP database.

Figure S1 shows the original, interpolated and extrapolated rarefaction curves of the different samples at different annealing temperatures for all bioreactors sampled in the study.

Figure S2 provides the Bray-Curtis based cluster analysis of samples at different annealing temperatures for all bioreactors sampled: a) accounting for all OTUs, and b) of >1% OTUs.

Figure S3 shows the phylogeny-dependent cluster analysis of samples at different annealing temperatures for all bioreactors sampled.

Figure 4 shows the phylogeny-based principal coordinates analysis of all annealing temperatures tested for each bioreactor sampled.

  1. Supplementary Material