454-Pyrosequencing Analysis of Bacterial Communities from Autotrophic Nitrogen Removal Bioreactors Utilizing Universal Primers: Effect of Annealing Temperature

Table 4

Ratings for the comparison of species richness for samples from different bioreactors at each annealing temperature. Higher mean ratio is related to higher performance of the annealing temperature.

Temperature	Mean ratio individuals	Mean ratio (int)	Mean ratio (ext)
44°C	89.36%	32.89%	31.00%
45°C	72.36%	88.05%	91.86%
46°C	41.79%	86.87%	72.43%
47°C	24.22%	93.29%	76.70%
48°C	37.09%	93.51%	84.76%
49°C	12.49%	92.73%	63.01%