Alleviation of Kainic Acid-Induced Brain Barrier Dysfunction by 4-O-Methylhonokiol in In Vitro and In Vivo Models

Figure 6

Effect of MH and scavengers on cld-5 levels in KA-treated hippocampal neuron. Immunoblots of lysed mice hippocampus 2 days following administration of MH or KA are shown. Neurons were exposed to KA at concentrations of 0, 10, 20, and 30 mg/kg for 48 h. Neurons were exposed to 10 mg/kg of KA at 0.5–1 h after 5–20 mg/kg of MH, trolox (50 mg/kg, i.p.), and DMTU (50 mg/kg, i.p.) pretreatment. GAPDH levels were measured to confirm equal protein loading.

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