Research Article
Alleviation of Kainic Acid-Induced Brain Barrier Dysfunction by 4-O-Methylhonokiol in In Vitro and In Vivo Models
Figure 9
Reduction activity of MH on •OH. (a) ESR spectra of DMPO/•OH adduct were generated in a Fenton Reaction System. The solutions with a final volume of 0.1 mL contained 2 mM ferrous sulfate, 2 mM H2O2, and 100 mM phosphate buffer (pH 7.2). Reactions were started by addition of ferrous ammonium sulfate (2 mM final concentration) and the steady-state ESR were recorded at 15 min after the Fenton Reaction. Line A; DMPO/•OH adduct, B; MH (1 mg/mL), C; trolox (1 mM), and D; DMTU (1 mM). (b) Time course of •OH degradation induced by Fenton Reaction System. DMPO/•OH adduct, MH (1 mg/mL), trolox (1 mM), and DMTU (1 mM).
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