A Versatile Orthotopic Nude Mouse Model for Study of Esophageal Squamous Cell Carcinoma

Figure 4

AKT knockdown. (a) AKT knockdown (KD) Western blots of three ESCC cell lines indicates successful AKT knockdown. (b) In vitro real-time invasion assays show significant inhibition in all three ESCC cell lines between vector alone (VA) and AKT KD; indicates . (c) Quantitative PCR data shows inhibition in AKT downstream angiogenesis markers, IL8 and VEGFA, in all three ESCC cell lines after AKT KD. (d) Histogram of incidence take rate, which is in terms of detectable luciferin signals after defined exposure taken at medium field in one minute, of the orthotopic assay demonstrates significant tumor growth inhibition comparing the combined AKT KD groups (17/25, 68%) versus the control (12/12, 100%) (). Control (ctr) = scrambled oligonucleotide control.

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