Trigonella foenum (Fenugreek) Induced Apoptosis in Hepatocellular Carcinoma Cell Line, HepG2, Mediated by Upregulation of p53 and Proliferating Cell Nuclear Antigen
(a) Apoptosis enzyme-linked immunosorbent assay, ELISA. Apoptosis detection assay was applied to detect apoptosis induction in HepG2 cells after treatment with FCE. Cells were treated with (100~500 μg/mL range) FCE for 48 h. Cells without drug treatment were used as controls. as compared to the untreated controls, using the unpaired Student -test. Each data point is an average of three independent experiments and is expressed as M ± SD. (b) Flow cytometric analysis of HepG2 cells after treatment with or without Fenugreek seed crude extract (FCE). Cell cycle analysis shows % of cell cycle phases of HepG2 cell line treated with or without 100~500 μg/mL range of FCE for 48 h. as compared to the untreated controls, using the unpaired Student -test. Each data point was an average of results from three independent experiments performed in triplicate and presented as M ± SD.