(a) Fenugreek seed crude extract (FCE) upregulated p53 and PCNA expression levels in HepG-2 cells line, using western blot analysis. Cells were treated with or without (500 μg/mL) FCE for different time intervals. Our data showed a significant increase in p53 and PCNA protein expression, respectively. Blots were normalized for total protein loading. Representative data from three independent experiments are shown. (b) Apoptosis indicated by poly(ADP-ribose) polymerase (PARP) cleavage. Western blot analysis for poly(ADP-ribose) polymerase (upper panel) and Bax protein expression (lower panel). Cells were treated with or without 100–500 μg/mL range of Fenugreek seed crude extract, FCE, for 48 h. Blots were normalized for total protein loading.