BioMed Research International / 2015 / Article / Fig 5

Research Article

Trigonella foenum (Fenugreek) Induced Apoptosis in Hepatocellular Carcinoma Cell Line, HepG2, Mediated by Upregulation of p53 and Proliferating Cell Nuclear Antigen

Figure 5

(a) Fenugreek seed crude extract (FCE) upregulated p53 and PCNA expression levels in HepG-2 cells line, using western blot analysis. Cells were treated with or without (500 μg/mL) FCE for different time intervals. Our data showed a significant increase in p53 and PCNA protein expression, respectively. Blots were normalized for total protein loading. Representative data from three independent experiments are shown. (b) Apoptosis indicated by poly(ADP-ribose) polymerase (PARP) cleavage. Western blot analysis for poly(ADP-ribose) polymerase (upper panel) and Bax protein expression (lower panel). Cells were treated with or without 100–500 μg/mL range of Fenugreek seed crude extract, FCE, for 48 h. Blots were normalized for total protein loading.
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