TY - JOUR A2 - Mukherjee, Amitava AU - Pacor, Sabrina AU - Grillo, Alberto AU - Đorđević, Luka AU - Zorzet, Sonia AU - Lucafò, Marianna AU - Da Ros, Tatiana AU - Prato, Maurizio AU - Sava, Gianni PY - 2015 DA - 2015/05/19 TI - Effects of Two Fullerene Derivatives on Monocytes and Macrophages SP - 915130 VL - 2015 AB - Two fullerene derivatives (fullerenes 1 and 2), bearing a hydrophilic chain on the pyrrolidinic nitrogen, were developed with the aim to deliver anticancer agents to solid tumors. These two compounds showed a significantly different behaviour on human neoplastic cell lines in vitro in respect to healthy leukocytes. In particular, the pyrrolidinium ring on the fullerene carbon cage brings to a more active compound. In the present work, we describe the effects of these fullerenes on primary cultures of human monocytes and macrophages, two kinds of immune cells representing the first line of defence in the immune response to foreign materials. These compounds are not recognized by circulating monocytes while they get into macrophages. The evaluation of the pronecrotic or proapoptotic effects, analysed by means of analysis of the purinergic receptor P2X7 activation and of ROS scavenging activity, has allowed us to show that fullerene 2, but not its analogue fullerene 1, displays toxicity, even though at concentrations higher than those shown to be active on neoplastic cells. SN - 2314-6133 UR - https://doi.org/10.1155/2015/915130 DO - 10.1155/2015/915130 JF - BioMed Research International PB - Hindawi Publishing Corporation KW - ER -