Effects of Two Fullerene Derivatives on Monocytes and Macrophages

Table 2

Cell cycle analysis of fullerene derivatives U937 treated cells.

	% G1	% S	% G2M
24 h
Control	36.3 ± 0.7	59.9 ± 0.9	3.9 ± 0.2
Fullerene 1	35.7 ± 0.5	58.3 ± 0.3
Fullerene 2	37.0 ± 0.8
48 h
Control	46.2 ± 1.7	47.6 ± 1.3	6.2 ± 0.5
Fullerene 1	43.1 ± 0.7		6.3 ± 0.7
Fullerene 2			7.1 ± 0.5
72 h
Control	46.7 ± 0.7	47.5 ± 0.5	5.8 ± 0.5
Fullerene 1	46.4 ± 0.8	47.7 ± 0.6	5.9 ± 0.3
Fullerene 2			6.0 ± 0.3

Cells (1 × 10⁶/mL) were exposed for 24–72 hr long-lasting treatment with 10 M of fullerene 1 and fullerene 2 and then subjected to PI staining prior to performing the flow cytometric cell cycle analysis. The percentage of each phase reported was calculated by MCycle analysis software. , , and versus untreated controls; , versus fullerene 1; Student-Newman-Keuls multiple comparisons test, ANOVA.