Molecular Detection of Equine Herpesvirus Types 1 and 4 Infection in Healthy Horses in Isfahan Central and Shahrekord Southwest Regions, Iran

Table 1

Primer sequence used for detection of EHV-1 and EHV-4 genes in horses blood.

Virus	Primer sequence	Size (base pair)	Annealing temperature	GenBank accession numbers
EHV-1	F: 5′-GCAAACAACAGAGGGTCGATAGAAG-3′	342	65°C	JQ692316
	R: 5′-GTCGATGTCGTAAAACCTGAGAG-3′
EHV-4	F: 5′-TATTGTTTCCGCCACTCTTGACG-3′	508	66°C	JX416462
	R: 5′-GTAGAATCGGAGGGCGTGAAGC-3′

Key: EHV-1: equine herpesvirus type 1; EHV-4: equine herpesvirus type 4.