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BioMed Research International
Volume 2015, Article ID 926513, 13 pages
http://dx.doi.org/10.1155/2015/926513
Research Article

Antimicrobial Activity Evaluation on Silver Doped Hydroxyapatite/Polydimethylsiloxane Composite Layer

1National Institute for Materials Physics, P.O. Box MG 07, 077125 Magurele, Romania
2National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, P.O. Box MG 36, 077125 Magurele, Romania
3Faculty of Physics, University of Bucharest, 405 Atomistilor Street, P.O. Box MG1, 077125 Magurele, Romania
4Horiba Jobin Yvon SAS, 16-18 Rue du Canal, 91165 Longjumeau Cedex, France
5Microbiology Department, Faculty of Biology, University of Bucharest, 1–3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania
6Earth, Environmental and Life Sciences Section, Research Institute of the University of Bucharest, 1–3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania
7Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania

Received 14 April 2015; Accepted 26 May 2015

Academic Editor: Julietta V. Rau

Copyright © 2015 C. S. Ciobanu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The goal of this study was the preparation, physicochemical characterization, and microbiological evaluation of novel hydroxyapatite doped with silver/polydimethylsiloxane (Ag:HAp-PDMS) composite layers. In the first stage, the deposition of polydimethylsiloxane (PDMS) polymer layer on commercially pure Si disks has been produced in atmospheric pressure corona discharges. Finally, the new silver doped hydroxyapatite/polydimethylsiloxane composite layer has been obtained by the thermal evaporation technique. The Ag:HAp-PDMS composite layers were characterized by various techniques, such as Scanning Electron Microscopy (SEM), Glow Discharge Optical Emission Spectroscopy (GDOES), and X-ray photoelectron spectroscopy (XPS). The antimicrobial activity of the Ag:HAp-PDMS composite layer was assessed against Candida albicans ATCC 10231 (ATCC—American Type Culture Collection) by culture based and confirmed by SEM and Confocal Laser Scanning Microscopy (CLSM) methods. This is the first study reporting the antimicrobial effect of the Ag:HAp-PDMS composite layer, which proved to be active against Candida albicans biofilm embedded cells.