Gene Delivery into Plant Cells for Recombinant Protein Production

Figure 1

Transgene delivery by agroinfiltration into N. benthamiana and lettuce plants. Agrobacteria carrying the expression cassette of GFP or DsRed in geminiviral vectors were syringe-infiltrated into a N. benthamiana leaf (a) and GFP or DsRed expression was observed 4 days after infiltration under UV light (b). Similarly, A. tumefaciens cells harboring the expression cassette of GFP in a geminiviral vector were vacuum infiltrated into a lettuce plant (c) and GFP expression was examined 4 days after infiltration (d). The yellow spot in (b) indicates the leaf area that was coinfiltrated with agrobacteria carrying the expression cassette of GFP and DsRed.

(a)

(b)

(c)

(d)