Gene Delivery into Plant Cells for Recombinant Protein Production
Transgene delivery by agroinfiltration into N. benthamiana and lettuce plants. Agrobacteria carrying the expression cassette of GFP or DsRed in geminiviral vectors were syringe-infiltrated into a N. benthamiana leaf (a) and GFP or DsRed expression was observed 4 days after infiltration under UV light (b). Similarly, A. tumefaciens cells harboring the expression cassette of GFP in a geminiviral vector were vacuum infiltrated into a lettuce plant (c) and GFP expression was examined 4 days after infiltration (d). The yellow spot in (b) indicates the leaf area that was coinfiltrated with agrobacteria carrying the expression cassette of GFP and DsRed.