Comparison of Different Strategies for Selection/Adaptation of Mixed Microbial Cultures Able to Ferment Crude Glycerol Derived from Second-Generation Biodiesel

Figure 5

Results of batch transfers, during the enrichment of anaerobic sludge, showing H2% (a) in the headspace, soluble metabolites from nonpretreated anaerobic sludge (b), and soluble metabolites from heat-shock treated anaerobic sludge (c). MM-KC = Minimal Medium with Kinetic Control (21 h); MM-EF = Minimal Medium with End of Fermentation (72 h); BA-KC = Basal Medium with Kinetic Control (21 h); BA-EF = Basal Medium with End of Fermentation (72 h).