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BioMed Research International
Volume 2015, Article ID 938721, 11 pages
Research Article

Detection of Tropical Fungi in Formalin-Fixed, Paraffin-Embedded Tissue: Still an Indication for Microscopy in Times of Sequence-Based Diagnosis?

1Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Bernhard-Nocht Street 74, 20359 Hamburg, Germany
2Institute for Microbiology, Virology and Hygiene, University Medicine Rostock, Schillingallee 70, 18057 Rostock, Germany
3Central Laboratory Department/Department of Clinical Chemistry, University Medicine Goettingen, Robert Koch Street 40, 37075 Göttingen, Germany
4Department of Infectious Disease Pathology, Bernhard Nocht Institute for Tropical Medicine Hamburg, Bernhard Nocht Street 74, 20359 Hamburg, Germany
5LADR GmbH MVZ Dr. Kramer & Kollegen, Lauenburger Straße 67, 21502 Geesthacht, Germany

Received 21 October 2014; Revised 27 February 2015; Accepted 9 March 2015

Academic Editor: Rosely Zancope-Oliveira

Copyright © 2015 Hagen Frickmann et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Altogether, 6 supplementary materials are presented to provide further details regarding the applied methods and the observed results.

Supplementary material 1 describes the applied pan-fungal PCR protocols, the inhibition control PCR and the sample quality assessment PCR in detail.

The supplementary materials 2-6 are an addition to table 3 of the paper. They provide more detailed information regarding the observed ct-values, melting curve peaks (if applicable), detection of visible bands in electrophoresis (if applicable), readable sequence fragment lengths, coverage and sequence identities of pan-fungal PCRs 1-5. Thereby, they allow for a better interpretation of PCR and sequencing results.

  1. Supplementary Material