BioMed Research International / 2015 / Article / Tab 2 / Research Article
Detection of Tropical Fungi in Formalin-Fixed, Paraffin-Embedded Tissue: Still an Indication for Microscopy in Times of Sequence-Based Diagnosis? Table 2 Results of inhibition control PCR (phocid herpesvirus PCR), DNA extraction control PCR (18S rRNA gene PCR), and specific PCR/sequencing results for Histoplasma capsulatum and Mucorales. Ct = cycle threshold. N.a. = not applicable.
Sample I.D. Histological diagnosis Ct-value of phocid herpesvirus PCR Ct-value of human 18S rRNA gene PCR DNA concentration as measured by Picodrop Histoplasma -specific nested PCR (and consecutive sequencing)Mucorales-specific seminested PCR (and consecutive sequencing) Case 1 Chromoblastomycosis 22 23 12.3 ng/µ L N.a. N.a. Case 2 Mucormycosis 18 15 14.6 ng/µ L Negative Negative Case 3 Histoplasmosis 18 14 246.3 ng/µ L Negative Negative Case 4 Mucormycosis 20 — 26.9 ng/µ L Negative Positive (100% Lichtheimia /Absidia corymbifera ) Case 5 Histoplasmosis or Cryptococcosis 20 19 157.6 ng/µ L Negative Negative Case 6 Chromoblastomycosis 26 30 414.5 ng/µ L N.a. N.a. Case 7 Rhinosporidiosis 26 23 62.7 ng/µ L N.a. N.a. Case 8 Mycetoma/maduromycosis 22 33 618.9 ng/µ L N.a. N.a. Case 9 Rhinosporidiosis 18 22 21.9 ng/µ L N.a. N.a. Case 10 Mycetoma/maduromycosis 25 15 16.8 ng/µ L N.a. N.a. Case 11 Histoplasmosis 21 30 104.6 ng/µ L Negative Negative Case 12 Histoplasmosis 24 15 44.3 ng/µ L Negative Positive (no interpretable sequence results due to poor sequence quality) Case 13 Chromoblastomycosis 23 14 33.8 ng/µ L N.a. N.a. Case 14 Histoplasmosis 20 17 175.5 ng/µ L Positive (99% Histoplasma capsulatum ) Negative Case 15 Coccidioidomycosis 24 21 16.8 ng/µ L N.a. N.a. Case 16 Coccidioidomycosis 24 14 107.5 ng/µ L N.a. N.a. Case 17 Mycetoma/maduromycosis 18 13 26.3 ng/µ L N.a. N.a. Control 1 Negative control 22 34 609.7 ng/µ L N.a. N.a. Control 2 Negative control 23 25 655.3 ng/µ L N.a. N.a. Control 3 Negative control 19 31 676.6 ng/µ L N.a. N.a.