Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus

Table 2

Analysis of SSR markers in EST sequences of five families in Polyporales.

Nucleotide repeat	Ganodermataceae	Coriolaceae	Phanerochaetaceae	Polyporaceae	Total
Mono-
Number	6530	1607	41	2	8180
Number /Mb	200	53	289	19	821
%	79.8	19.6	0.5	0.02	100
Di-
Number	113	145	1	1	260
Number /Mb	3	5	7	10	25
%	43.46	55.77	0.38	0.38	100
Tri-
Number	386	430	—	—	816
Number /Mb	12	14	—	—	26
%	47.3	52.7	—	—	100
Tetra-
Number	8	9	—	—	17
Number /Mb	0.24	0.29	—	—	0.53
%	47.1	52.9	—	—	100
Penta-
Number	82	12	—	—	94
Number /Mb	2.51	0.39	—	—	2.9
%	87.23	12.77	—	—	100
Hexa-
Number	69	84	—	—	153
Number /Mb	2.1	2.7	—	—	4.8
%	45.1	54.9	—	—	100
Total
Number	7188	2287	42	3	9520
Number /Mb	113.12	36	0.66	0.05	149.81
%	75.5	24.02	0.44	0.03	100

Notes: no SSR sites were found in the EST sequences of Meruliaceae.