Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus
Table 9
The 23 selected EST-SSR primers that could amplify PCR products by using Polyporus umbellatus DNA as template.
Primera
Sequence (5′ to 3′)
GenBank access number
Tm value (°C)
Repeat motif
GL01
CTGCTCGTTGTACACGCTTC
HO719308.1
56
(GCA)7
GAAAGACAGACGCGGGATTA
GL02
GGTAATCTCGGCTTCACGAT
—
55
—
TTGAGAATCTTCGGCACCTC
GL03
AGTACGTAGCTGCCGTCCAG
—
58
—
CCTGATACGTCCCGTAACCA
GL09
GAGCTATCCAATCTATCGCCA
GO447546.1
55
(A)40⋯(A)29
GATACCCCTGCTTTGAGTCC
GL10
CTGACGACGTTAACCTAGGC
GO447482.1
53
(A)30⋯(A)32
GCGTTGATACCCCTGCTAC
GL11
ACCTCACTCTGTACTTATCCAC
GO447246.1
55
(G)10⋯(A)10
CCCATTGGCCGAAAAGCTTA
GL23
CGTCCCAGCAGCCGAGTCC
HO727333.1
63
(GCA)9⋯(CAG)9
CCGCGACTCCAGGCCCGAA
PS01
CTCGACGCATCAAATCACTCC
JK479811.1
55
(TC)5⋯(CCA)6
ACCAGCTTGATCATGGACGAG
PS02
GACGGGAAGGATCAGAACGAG
JK479807.1
55
(CAA)6
GTCGACGAGTGAGATGAGGG
PS05
CAACCGTCATCGATTTAACCC
JK479810.1
57
(ACT)6
CCTCAAGATCTCCAGCGGTCA
PS06
AAAGCATTCAAACAGCAGC
JK479808.1
55
(AAG)6
GTACTGGCGTGTTGATGTTGG
PS07
CAGCGGGCGTCTTTATTGTGT
JK479806.1
59
(CAA)6
GTTGGTCCCCTTGCTGCCCTG
PS08
CAAAGATCCAGCGTAACTCCC
JK479804.1
56
(TC)13
CAACAGTGTAGTGACCACGAG
PS09
TTCTTCACTCCATCCAGCCTT
JK479802.1
57
(CTA)6
TTGGCGAGGGAGTAGTAGCTG
PS10
ACTACTACTCCCACTACCACT
JK479802.1
57
(ACT)5
GATGCGAATAAGATGGACAGT
FP10
CTGGACACCCCGAACAACTGG
GR368360.1
58
(CAA)5
CGACACCGCCTCAGTTGCCAT
FP11
AGCGAGACTACAGCAGAGAC
GR369989.1
59
(GTC)6
ACAGAGGACGTGGTGGAAGG
FP12
TGCTCATCCACAACCGCCACC
GR370913.1
60
(CCA)6
CTCCTTTCGGCAAGACCCAGT
FP13
CACGTATCCCCATCCGCCAC
GR370248.1
60
(CG)7
GAGGAGGGGCAGGACGAGGG
FP14
GAACAAGACTGACCCCGCGTAT
GR370241.1
55
(CGA)5
CACCCCAACAGGCAACTCTC
FP15
CTGGACACCCCGAACAACTGG
GR370616.1
60
(CAA)5
CGACACCGCCTCAGTTGCCAT
FP16
GCCCCATCACATCGCGCTCT
GR368431.1
60
(CAA)5
GTTGCCGCTGCCATACCCACT
FP17
GCCGTCCGTGAAACCGTCCT
GR368155.1
60
(GAC)5
GACAAGCGCCGCAGAGTCCAG
GL, PS, and FP represented that these primers were designed from EST-SSR sites of Ganoderma lucidum, Puccinia striiformis f. sp. tritici, and Fomitopsis palustris, respectively.
